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Image Search Results
Journal: Journal of the American Heart Association
Article Title: Elastin‐Specific Autoimmunity in Smokers With Thoracic Aortic Aneurysm and Dissection is Independent of Chronic Obstructive Pulmonary Disease
doi: 10.1161/jaha.118.011671
Figure Lengend Snippet: Figure 1. EFs-specific CD4+ T cell response in TAA/TAD. A, Representative intracytoplasmic cytokine of CD4+ T cells (59105) isolated from peripheral blood of healthy controls (HC) or thoracic aortic aneurysm and dissection (TAA/TAD) patients were co-cultured with irradiated CD1a+/CD14+ APCs (59104) with EFs (3, 10, or 30 lg/mL) or no EFs. After 3 days, interferon-c and IL-17A producing CD4+ T cells were detected using flow cytometry. Relative abundance and mean fluorescent intensity (MFI) shown in the right lower quadrants. Data are representative of 4 to 5 subjects in each group. B, Representative histogram of EFs- specific CD4+ T cell proliferation. CFSE-labeled CD4+ T cells (59105) were cultured with APCs (59104) in presence of EFs (30 lg/mL) for 3 days. 2% phytohemagglutinin was used as positive control. Data are representative of 4 to 5 subjects in each group. C, Supernatants from the same co-culture conditions described in (A); concentration of interferon-c, IL-1b, IL-10, IL-17, and IL-13 were measured and plotted as fold change over nil stimulation. Each dot represents a data point from an individual subject. *P<0.05, and ***P<0.001 as determined by the Mann–Whitney test; HC: n=37; TAA/TAD n=49. APCs indicates antigen presenting cells; CFSE, carboxyfluorescein succinimidyl ester; EFs, elastin fragments; HC, healthy control; IFN-c, interferon-c; IL, interleukin; MFI, mean fluorescent intensity; PHA, phytohemagglutinin; TAA/TAD, thoracic aortic aneurysm and dissection.
Article Snippet: Approximately 10 to 309106 peripheral blood mononuclear cells were isolated from whole blood and were labeled with MicroBeads conjugated to
Techniques: Isolation, Dissection, Cell Culture, Irradiation, Cytometry, Labeling, Positive Control, Co-Culture Assay, Concentration Assay, MANN-WHITNEY, Control
Journal: Journal of the American Heart Association
Article Title: Elastin‐Specific Autoimmunity in Smokers With Thoracic Aortic Aneurysm and Dissection is Independent of Chronic Obstructive Pulmonary Disease
doi: 10.1161/jaha.118.011671
Figure Lengend Snippet: Figure 2. EFs-specific CD4+ T cell expression of interferon-c and IL-1b are independent of airway obstruction in TAA/TAD. Expression of interferon-c (A) and IL-1b (B) in response against EFs was assessed in healthy smoker and non-smoker HC (n=37), and smoker and non-smoker TAA/TAD patients (n=49) or TAA/ TAD without chronic obstructive airway disease (COPD; n=37). Each dot represents a data point from an individual subject. *P<0.05, **P<0.01, as determined by the Kruskal–Wallis test with Dunn post-test for multiple comparisons. COPD indicates chronic obstructive airway disease; EFs, elastin fragments; HC, healthy control; IFN-c, interferon-c; IL, interleukin; NS, not significant; TAA/TAD, thoracic aortic aneurysm and dissection.
Article Snippet: Approximately 10 to 309106 peripheral blood mononuclear cells were isolated from whole blood and were labeled with MicroBeads conjugated to
Techniques: Expressing, Control, Dissection
Journal: Journal of the American Heart Association
Article Title: Elastin‐Specific Autoimmunity in Smokers With Thoracic Aortic Aneurysm and Dissection is Independent of Chronic Obstructive Pulmonary Disease
doi: 10.1161/jaha.118.011671
Figure Lengend Snippet: Figure 3. EFs-specific CD4+ T cell expression of interferon-c, but not IL-1b response is dependent on history of smoking in TAA/TAD. Expression of interferon-c (A) and IL-1b (B) in response against EFs was assessed in HC non-smoker (n=18), smoker (former n=13, current n=6), TAA/TAD non-smoker (n=15), TAA/TAD smokers (former n=20, current n=14). Each dot represents a data point from an individual subject. For 2 groups comparison, Mann–Whitney test was used. For multiple comparisons in >3 groups, the Kruskal–Wallis test with Dunn’s post-test for multiple comparisons was used. *P<0.05, **P<0.01. EFs indicates elastin fragments; HC, healthy control; IFN-c, interferon-c; IL, interleukin; NS, not significant; TAA/ TAD, thoracic aortic aneurysm and dissection.
Article Snippet: Approximately 10 to 309106 peripheral blood mononuclear cells were isolated from whole blood and were labeled with MicroBeads conjugated to
Techniques: Expressing, Comparison, MANN-WHITNEY, Control, Dissection